6W1B

N-terminal domain of mouse surfactant protein B with bound lipid, Y59A/H79A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	90.335, 169.527, 73.714
Unit cell angles	90.00, 126.90, 90.00

Refinement procedure

Resolution	84.760 - 2.310
R-factor	0.2422
Rwork	0.241
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vyn lipid removed
RMSD bond length	0.002
RMSD bond angle	0.672
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.760	2.397
High resolution limit [Å]	2.310	2.310
Rmerge	0.084	2.030
Number of reflections	37983	3077
<I/σ(I)>	13.34	0.72
Completeness [%]	97.5	80.13
Redundancy	6.9	6
CC(1/2)	0.995	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293.15	100 mM Na/K phosphate pH 6.2, 200 mM NaCl, 47.5% (w/v) PEG 200