6W16

Crystal structure of a human metapneumovirus monomeric fusion protein complexed with 458 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-16
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	128.489, 128.489, 188.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.050 - 3.100
R-factor	0.1934
Rwork	0.190
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wb0
RMSD bond length	0.011
RMSD bond angle	1.257
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.141
Rmeas	0.144
Rpim	0.032
Number of reflections	31997	4679
<I/σ(I)>	17.62
Completeness [%]	100.0	100
Redundancy	21	20.9
CC(1/2)	0.999	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Lithium sulfate monohydrate