6W12
Crystal Structure of the Carbohydrate Recognition Domain of the Human Macrophage Galactose C-Type Lectin Bound to the Tumor-Associated Tn Antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2019-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9863 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.155, 52.155, 112.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.584 - 2.000 |
| Rwork | 0.168 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.080 | 0.280 |
| Number of reflections | 12784 | 1241 |
| <I/σ(I)> | 12.1 | 5.9 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 5.7 | 4.9 |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM Magnesium chloride, 100mM Tris, 2-3M NaCl |
