6W0R
Human 8-oxoguanine glycosylase interrogating fully intrahelical undamaged DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2013-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 88.928, 88.928, 210.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.500 - 2.350 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebm |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 2.470 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.170 | 0.989 |
| Number of reflections | 21524 | 3060 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM NH4NO3, and 20 % polyethylene glycol 3350 |
