6W0J

Closed-gate KcsA incubated in BaCl2/NaCl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	I 4
Unit cell lengths	156.329, 156.329, 76.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.612 - 2.500
R-factor	0.1914
Rwork	0.190
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.008
RMSD bond angle	0.957
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.612	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	32029	3625
<I/σ(I)>	7.1
Completeness [%]	99.9
Redundancy	9.6
CC(1/2)	0.990	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5