6VZV

TTLL6 bound to gamma-elongation analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	75.072, 109.716, 173.032
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	45.840 - 2.330
R-factor	0.183
Rwork	0.182
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylr
RMSD bond length	0.003
RMSD bond angle	0.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.840	2.414
High resolution limit [Å]	2.330	2.330
Rmerge	0.092	0.445
Rmeas	0.109	0.538
Rpim	0.059	0.298
Number of reflections	118734	11356
<I/σ(I)>	12.8	2.12
Completeness [%]	99.1	95.22
Redundancy	3.3	3.1
CC(1/2)	0.990	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	100 mM Sodium Citrate, pH 6.2, 200 mM MgCl2, 8-12% Peg 20000.