6VZT
TTLL6 bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.633, 75.515, 115.723 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 45.930 - 2.180 |
| R-factor | 0.2386 |
| Rwork | 0.237 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ylr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.030 | 0.279 |
| Rmeas | 0.030 | 0.395 |
| Rpim | 0.022 | 0.279 |
| Number of reflections | 105266 | 10288 |
| <I/σ(I)> | 14.14 | 2.49 |
| Completeness [%] | 99.7 | 98.59 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.999 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 200 mM Ammonium Sulfate, 0.1M Bis Tris pH 5.5, 16% Peg 3350 |
