6VZF

Crystal Structure of Atg11 Coiled-Coil 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979100
Spacegroup name	P 31 2 1
Unit cell lengths	103.105, 103.105, 37.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.610 - 2.030
R-factor	0.2273
Rwork	0.226
R-free	0.24590
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.719
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.650	44.650	2.080
High resolution limit [Å]	2.030	9.080	2.030
Rmerge	0.107	0.059	1.852
Rmeas	0.109	0.060	1.901
Rpim	0.024	0.014	0.427
Total number of observations	303942	3292	21698
Number of reflections	15063	189	1110
<I/σ(I)>	16.7	57.6	2
Completeness [%]	99.9	97.7	100
Redundancy	20.2	17.4	19.5
CC(1/2)	0.999	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	90 mM LiNaK, 0.1 M Buffer system 4 pH 6.5 (MOPSO + Bis Tris), 50% v/v precipitant mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol)
1	VAPOR DIFFUSION, HANGING DROP		295	90 mM LiNaK, 0.1 M Buffer system 4 pH 6.5 (MOPSO + Bis Tris), 50% v/v precipitant mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol)