6VZD

N-terminal domain of mouse surfactant protein B (K46E/R51E mutant) with bound lipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.793, 66.124, 59.620
Unit cell angles	90.00, 97.37, 90.00

Refinement procedure

Resolution	59.130 - 1.880
R-factor	0.1727
Rwork	0.170
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6VYN (lipid removed)
RMSD bond length	0.006
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.130	1.947
High resolution limit [Å]	1.880	1.880
Rmerge	0.100	1.511
Number of reflections	31237	2186
<I/σ(I)>	40.86	2.41
Completeness [%]	92.2
Redundancy	53.8
CC(1/2)	1.000	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293.15	100 mM citrate buffer pH 5.4, 23-25% PEG 4000, 14-15% 2-propanol