6VY9
Crystal structure of NotF prenyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 153.591, 208.295, 217.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.320 - 3.190 |
| R-factor | 0.3074 |
| Rwork | 0.307 |
| R-free | 0.31580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ld7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.300 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Number of reflections | 115959 | 11141 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 93.6 | |
| Redundancy | 7.5 | |
| CC(1/2) | 1.000 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 3.0 M NaCl, 60 mM urea, 0.1 M MES pH 6.7, 15% ethylene glycol |
