6VY4

Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV-32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-26
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	195.889, 84.490, 122.834
Unit cell angles	90.00, 95.93, 90.00

Refinement procedure

Resolution	49.506 - 2.000
R-factor	0.1853
Rwork	0.183
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vsm
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.743
Number of reflections	134705	19582
<I/σ(I)>	11.7
Completeness [%]	100.0
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.5 M ammonium sulfate, 5% isopropanol