6VXT
Activated Nitrogenase MoFe-protein from Azotobacter vinelandii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.535, 203.435, 83.857 |
| Unit cell angles | 90.00, 103.94, 90.00 |
Refinement procedure
| Resolution | 41.710 - 1.740 |
| R-factor | 0.1726 |
| Rwork | 0.172 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u7q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmeas | 0.150 | |
| Rpim | 0.060 | 0.970 |
| Number of reflections | 263634 | 23896 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 96.8 | 71.9 |
| Redundancy | 6.1 | 4 |
| CC(1/2) | 0.989 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 14-19 % w/v PEG smear high, 0.16 M ammonium acetate, 0.1 M sodium citrate(pH 5.0), 25.6 % v/v glycerol, 5 mM sodium dithionite |
