6VWP
Crystal structure of E. coli guanosine kinase in complex with ppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 245.949, 245.949, 221.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.160 - 3.450 |
| R-factor | 0.2008 |
| Rwork | 0.200 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vwo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.510 |
| High resolution limit [Å] | 3.420 | 9.360 | 3.450 |
| Rmerge | 0.117 | 0.047 | 1.750 |
| Rmeas | 0.121 | 0.048 | 1.810 |
| Rpim | 0.029 | 0.012 | 0.461 |
| Number of reflections | 103948 | 5402 | 5193 |
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 99.8 | 98.5 | 100 |
| Redundancy | 16.7 | 15.9 | 15.3 |
| CC(1/2) | 0.999 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M sodium acetate pH 4.8, 3.1M sodium formate |
