6VW8
Formate Dehydrogenase FdsABG subcomplex FdsBG from C. necator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.656, 127.050, 99.840 |
| Unit cell angles | 90.00, 123.50, 90.00 |
Refinement procedure
| Resolution | 48.534 - 2.300 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.22310 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.056 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.534 | 48.530 | 2.360 |
| High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
| Rmerge | 0.129 | 0.066 | 0.462 |
| Rmeas | 0.141 | 0.072 | 0.514 |
| Rpim | 0.056 | 0.029 | 0.223 |
| Total number of observations | 4225 | 23427 | |
| Number of reflections | 61209 | 710 | 4497 |
| <I/σ(I)> | 8.9 | 17.7 | 3.3 |
| Completeness [%] | 99.9 | 99 | 99.5 |
| Redundancy | 6.1 | 6 | 5.2 |
| CC(1/2) | 0.985 | 0.994 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2-propanol, ammonium sulfate, HEPES-NaOH, in an anaerobic chamber |
