6VW1
Structure of SARS-CoV-2 chimeric receptor-binding domain complexed with its receptor human ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.435, 118.034, 112.075 |
Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
Resolution | 59.020 - 2.680 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ajf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.597 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.030 | 2.780 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.081 | 1.470 |
Number of reflections | 58219 | 5774 |
<I/σ(I)> | 12.08 | |
Completeness [%] | 96.7 | 98.97 |
Redundancy | 3.9 | 4 |
CC(1/2) | 0.998 | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris, PEG 6000, and 100 mM NaCl. |