6VVR

Q0 fused 4-OT wild type symmetric trimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 43
Unit cell lengths	72.943, 72.943, 82.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.840 - 1.800
Rwork	0.172
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5unq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.840	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	39332	3844
<I/σ(I)>	11.5
Completeness [%]	98.8	96.6
Redundancy	5.6
CC(1/2)	0.996	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298.15	0.2 M ammonium sulfate, 27-33% PEG4000