6VVH
Arabidopsis thaliana dihydrodipicolinate synthase isoform 1 (DHDPS1) in complex with lysine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.044, 97.626, 176.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 1.792 |
| Rwork | 0.166 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dpp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.931 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 153659 | 6989 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES (pH 7.5), 0.1 M NaCl, and 1.4 M (NH4)2SO4 |
