6VVG
Structure of the Cydia pomonella Granulovirus kinase, PK-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.937, 115.370, 119.395 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.570 - 2.010 |
R-factor | 0.1946 |
Rwork | 0.193 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v70 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.648 |
Data reduction software | XDS |
Data scaling software | Aimless (7.0) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.940 | 41.940 | 2.060 |
High resolution limit [Å] | 2.010 | 8.990 | 2.010 |
Rmerge | 0.294 | 0.057 | 1.307 |
Rmeas | 0.306 | 0.061 | 1.395 |
Rpim | 0.085 | 0.019 | 0.477 |
Total number of observations | 486841 | 5809 | 18803 |
Number of reflections | 38430 | 530 | 2276 |
<I/σ(I)> | 9.6 | 29.8 | 1.6 |
Completeness [%] | 97.3 | 99.5 | 80.6 |
Redundancy | 12.7 | 11 | 8.3 |
CC(1/2) | 0.993 | 0.998 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 300 mM Ammonium chloride, 16.5 % (w/v) PEG 3350 and 1 mM AMP |