6VUP

Reverse Transcriptase Diabody with R83T/E85C Mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	72.942, 86.686, 37.078
Unit cell angles	90.00, 116.23, 90.00

Refinement procedure

Resolution	43.340 - 1.400
R-factor	0.1332
Rwork	0.131
R-free	0.17060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vrp
RMSD bond length	0.007
RMSD bond angle	0.927
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.041	0.049
Rmeas	0.049	0.301
Number of reflections	39530	1905
<I/σ(I)>	24.7
Completeness [%]	97.4
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.4	293	6 % v/v 2-Propanol, 0.1 M Sodium Acetate trihydrate pH 4.5, 26% PEG 550 MME