6VU7

Crystal structure of YbjN, a putative transcription regulator from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.462, 110.891, 59.924
Unit cell angles	90.00, 116.71, 90.00

Refinement procedure

Resolution	29.930 - 2.590
R-factor	0.2403
Rwork	0.237
R-free	0.29460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fr7
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.590	2.600
Rmerge	0.126	0.597
Rpim	0.083	0.536
Number of reflections	19643	961
<I/σ(I)>	13.59	2.68
Completeness [%]	99.7
Redundancy	3.2
CC(1/2)		0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	298	0.1 M diammionium citrate, 0.1 M MES pH 5.2, 26% (w/v) PEG3350, 2% isopropanol