6VTX

Crystal structure of human KLF4 zinc finger DNA binding domain in complex with NANOG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	77
Detector technology	CCD
Collection date	2019-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	38.964, 46.688, 45.200
Unit cell angles	90.00, 113.50, 90.00

Refinement procedure

Resolution	41.450 - 2.140
R-factor	0.2184
Rwork	0.217
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wbu
RMSD bond length	0.003
RMSD bond angle	0.403
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.210
High resolution limit [Å]	2.140	2.140
Number of reflections	8237	816
<I/σ(I)>	4.31
Completeness [%]	96.1
Redundancy	3.9
CC(1/2)	0.975	0.304

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.2 M Sodium iodide (pH 7.0) and 20% w/v polyethylene glycol 3,350