6VTO

Crystal structure of human Galectin-7 in complex with 4-O-beta-D-Galactopyranosyl-D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-18
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	30.520, 77.240, 111.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.190 - 1.690
R-factor	0.1845
Rwork	0.183
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.190	1.750
High resolution limit [Å]	1.690	1.690
Rmerge	0.086	0.836
Rmeas	0.093	0.901
Total number of observations	206559
Number of reflections	30152	2930
<I/σ(I)>	15.7	3
Completeness [%]	99.1	99.25
Redundancy	6.85	7.17
CC(1/2)	0.999	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.1 M Sodium Chloride, 0.1 M Tris pH 8, 20 % PEG3350, 5 % Glycerol