6VTC

p53-specific T cell receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	41.123, 106.369, 112.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.830
R-factor	0.1923
Rwork	0.190
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3utp 3vxt
RMSD bond length	0.016
RMSD bond angle	2.162
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.300	1.900
High resolution limit [Å]	1.830	1.830
Rmerge	0.081	0.328
Number of reflections	44465	4396
<I/σ(I)>	30.6
Completeness [%]	99.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295	20% (w/v) PEG 4000, 0.2 M LiSO4, 0.1 M MES