6VST
Arginase from Medicago truncatula in complex with ornithine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-03 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.672, 166.074, 150.875 |
Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
Resolution | 41.520 - 2.120 |
R-factor | 0.1599 |
Rwork | 0.160 |
R-free | 0.19910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vss |
RMSD bond length | 0.007 |
RMSD bond angle | 0.851 |
Data reduction software | XDS (VERSION Jan 26, 2018) |
Data scaling software | XSCALE (VERSION Jan 26, 2018) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.520 | 2.250 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.056 | 0.714 |
Number of reflections | 231608 | 36997 |
<I/σ(I)> | 15.5 | 1.9 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 4.2 | 4 |
CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 55 mM CaCl2, 55 mM MgCl2, 80 mM HEPES/MOPS buffer at pH 7.5, 30% ethylene glycol, 15 % polyethylene glycol 8000; cocrystallization with 50 mM L-arginine |