6VSS
Arginase from Medicago truncatula
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.331, 142.907, 90.045 |
| Unit cell angles | 90.00, 115.90, 90.00 |
Refinement procedure
| Resolution | 44.550 - 1.930 |
| R-factor | 0.1825 |
| Rwork | 0.182 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lhl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.893 |
| Data reduction software | XDS (VERSION May 1, 2016) |
| Data scaling software | XSCALE (VERSION May 1, 2016) |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.550 | 2.040 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.075 | 0.626 |
| Number of reflections | 130001 | 20449 |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 95.7 | 93.6 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 55 mM CaCl2, 55 mM MgCl2, 80 mM HEPES/MOPS buffer at pH 7.5, 30% ethylene glycol, 15 % polyethylene glycol 8000 |
