6VRP

Reverse Transcriptase Diabody with R83T Mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	52.728, 55.460, 157.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 1.600
R-factor	0.1474
Rwork	0.146
R-free	0.18270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x4x
RMSD bond length	0.004
RMSD bond angle	0.751
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.046	0.368
Rmeas	0.050	0.398
Number of reflections	61848	3029
<I/σ(I)>	39.14
Completeness [%]	99.9	100
Redundancy	6.6	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M Ammonium Sulfate, 20% PEG 3350