6VRM
T cell receptor-p53-HLA-A2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.405, 120.608, 119.693 |
| Unit cell angles | 90.00, 108.38, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.610 |
| R-factor | 0.1949 |
| Rwork | 0.191 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d2l 3sjv 2ak4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.752 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 2.700 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.044 | 0.343 |
| Number of reflections | 36118 | 3641 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.4 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | 20% (w/v) polyacrylic acid 5100, 0.1 M HEPES, 20 mM MgCl2 |
