6VPW

1.90 Angstrom Resolution Crystal Structure Chemotaxis protein CheX from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41
Unit cell lengths	44.093, 44.093, 143.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.580 - 1.900
R-factor	0.1766
Rwork	0.174
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h2d
RMSD bond length	0.006
RMSD bond angle	1.333
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.791
Rmeas	0.063	0.887
Rpim	0.028	0.397
Number of reflections	21375	1065
<I/σ(I)>	25	2.3
Completeness [%]	99.9	100
Redundancy	5	4.8
CC(1/2)		0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	Protein: 10.4 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3, Screen: Classics II (D7), 0.1M Bis-Tris pH 6.5, 25% (w/v) PEG 3350