6VPR

Crystal structure of the C-terminal domain of DENR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.008
Spacegroup name	P 64 2 2
Unit cell lengths	116.600, 116.600, 75.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.980 - 2.200
R-factor	0.227
Rwork	0.224
R-free	0.28770
Structure solution method	SAD
Data reduction software	XSCALE
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.980	100.980	2.260
High resolution limit [Å]	2.200	9.850	2.200
Rmerge	0.168	0.030	12.070
Rmeas	0.173	0.030	12.383
Total number of observations	596862
Number of reflections	28988	332	2131
<I/σ(I)>	11.71	65.18	0.14
Completeness [%]	100.0	99.7	100
Redundancy	20.59	20.066	19.897
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.2 M K/Na tartrate, 2.5 M Ammonium Sulfate, 0.1 M tri-Sodium citrate, pH 5.6