6VPB
A novel membrane-bound 6-phosphogluconate dehydrogenase from the acetic acid bacteria Gluconacetobacter diazotrophicus (Gd6PGD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-11 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.760, 82.097, 81.831 |
| Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
| Resolution | 36.480 - 1.870 |
| R-factor | 0.1838 |
| Rwork | 0.183 |
| R-free | 0.22220 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.480 |
| High resolution limit [Å] | 1.870 |
| Rmeas | 0.097 |
| Rpim | 0.023 |
| Number of reflections | 48489 |
| <I/σ(I)> | 51.5 |
| Completeness [%] | 99.2 |
| Redundancy | 16.5 |
| CC(1/2) | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | 10 mM sodium phosphate pH 6.0, 0.1 % triton X-100 |
