6VP4

Ethylene forming enzyme (EFE) in complex with Fe(II), L-arginine, and 2OG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-18
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	49.730, 78.807, 195.905
Unit cell angles	90.00, 92.67, 90.00

Refinement procedure

Resolution	40.770 - 1.830
R-factor	0.1748
Rwork	0.174
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lsq
RMSD bond length	0.006
RMSD bond angle	0.732
Data scaling software	HKL-2000
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.770	1.890
High resolution limit [Å]	1.830	1.830
Rpim	0.031	0.301
Number of reflections	132130	12759
<I/σ(I)>	18.82
Completeness [%]	98.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris propane, 0.1-0.2 M sodium chloride, 24-30% PEG6000