6VN5
USP7 IN COMPLEX WITH LIGAND COMPOUND 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.430, 65.078, 85.189 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.900 |
| R-factor | 0.2157 |
| Rwork | 0.213 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.020 | 3.150 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.064 | 0.443 |
| Rmeas | 0.074 | 0.034 |
| Number of reflections | 17172 | 3727 |
| <I/σ(I)> | 15.68 | 3.55 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 4 | 3.8 |
| CC(1/2) | 0.998 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 |
