6VM7

SILv44 T cell receptor bound to HLA-A2 presenting gp100 peptide (ITDQVPFSV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	68.118, 94.403, 143.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.460 - 2.410
R-factor	0.2015
Rwork	0.199
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tvh
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.410	2.410
Rmerge	0.126	0.566
Rmeas	0.138	0.629
Rpim	0.055	0.267
Number of reflections	35998	1725
<I/σ(I)>	13.1	2
Completeness [%]	98.8	97
Redundancy	6	5.1
CC(1/2)	0.990	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	16% v/v PEG3350, 280 mM ammonium citrate dibasic