6VM5
Structure of Moraxella osloensis Cap4 SAVED/CARF-domain containing receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-05-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99998 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 157.593, 157.593, 63.318 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.360 - 2.350 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unpublished Se-Met version of the same protein determied by SAD phasing |
RMSD bond length | 0.006 |
RMSD bond angle | 0.710 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SOLVE |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.360 | 2.430 |
High resolution limit [Å] | 2.340 | 2.340 |
Rpim | 0.032 | 0.895 |
Number of reflections | 37860 | 3455 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 99.2 | 92.4 |
Redundancy | 11.2 | 10.6 |
CC(1/2) | 0.999 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 110 mM Tris-HCl pH 8.5, 21% PEG-400 |