6VLW

Crystal Structure of 426cOD in Complex with VRC01 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	137.367, 137.367, 66.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.570 - 3.420
R-factor	0.2128
Rwork	0.210
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ifa
RMSD bond length	0.004
RMSD bond angle	0.663
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.480
High resolution limit [Å]	3.420	3.420
Number of reflections	15995	854
<I/σ(I)>	25.57
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.992	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18.425% PEG 3350, 11.011% 2-Methyl-2,4-pentanediol, 0.22M Lithium Sulfate, 0.11M Imidazole pH 6.5