6VLG

Crystal structure of mouse alpha 1,6-fucosyltransferase, FUT8 bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 65 2 2
Unit cell lengths	150.820, 150.820, 472.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.367 - 2.500
R-factor	0.1844
Rwork	0.182
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2de0
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.157	0.713
Rmeas	0.164	0.772
Rpim	0.049	0.279
Total number of observations	1135283	28905
Number of reflections	109145	4637
<I/σ(I)>	9.5	2
Completeness [%]	98.8	85.8
Redundancy	10.4	6.2
CC(1/2)	0.995	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	12% PEG 3350, 0.25 M NH4SO4, 0.1 M Tris, pH 7.4