6VLE
Crystal structure of human alpha 1,6-fucosyltransferase, FUT8 in its Apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.019, 62.204, 109.905 |
| Unit cell angles | 90.00, 90.88, 90.00 |
Refinement procedure
| Resolution | 47.251 - 2.280 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2de0 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.135 | 1.711 |
| Rmeas | 0.151 | 1.907 |
| Rpim | 0.067 | 0.833 |
| Total number of observations | 293792 | 23265 |
| Number of reflections | 58867 | 4549 |
| <I/σ(I)> | 8.2 | 1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 | 5.1 |
| CC(1/2) | 0.997 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 12% PEG 20000, 0.1 M HEPES, pH 7.5 |
