6VK7

Crystal Structure of reduced Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	62.458, 290.452, 139.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.110 - 2.120
R-factor	0.1644
Rwork	0.162
R-free	0.20330
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mhz
RMSD bond length	0.006
RMSD bond angle	0.773
Data reduction software	XDS
Data scaling software	PHENIX
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.110	2.160
High resolution limit [Å]	2.120	2.120
Rmerge	0.161
Rpim		0.983
Number of reflections	72012	4337
<I/σ(I)>	6.7
Completeness [%]	99.5
Redundancy	5.1
CC(1/2)	0.994	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tacsimate and PEG3350