6VK6

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	63.071, 292.634, 141.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.010 - 1.520
R-factor	0.1473
Rwork	0.146
R-free	0.16360
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mhz
RMSD bond length	0.005
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	PHENIX
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.790	1.550
High resolution limit [Å]	1.520	1.520
Rmerge	0.069
Rpim		0.692
Number of reflections	395118	9633
<I/σ(I)>	11.6
Completeness [%]	98.5
Redundancy	3.2
CC(1/2)	0.998	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tacsimate, PEG3350