6VK5

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	102.615, 105.459, 299.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.540 - 1.860
R-factor	0.1711
Rwork	0.170
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mhy 2mob
RMSD bond length	0.007
RMSD bond angle	0.857
Data reduction software	XDS
Data scaling software	PHENIX
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.460	1.890
High resolution limit [Å]	1.860	1.860
Rmerge	0.132
Rpim		0.816
Number of reflections	271196	13328
<I/σ(I)>	8.2
Completeness [%]	99.8	99.8
Redundancy	5
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tacsimate, 20% w/v PEG3350)