6VJO

Human parainfluenza virus type 3 fusion glycoprotein N-terminal heptad repeat domain+alpha/beta-VI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-01
Detector	BRUKER SMART 6000
Wavelength(s)	1.542
Spacegroup name	H 3
Unit cell lengths	48.345, 48.345, 134.546
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.550 - 2.000
R-factor	0.2021
Rwork	0.200
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ztm
RMSD bond length	0.005
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.550	2.130
High resolution limit [Å]	2.000	2.000
Rmeas	0.171	0.944
Rpim	0.058	0.478
Number of reflections	7675	1107
<I/σ(I)>	10.5	2.4
Completeness [%]	96.8	83.9
Redundancy	8.7
CC(1/2)	0.996	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)