6VJC
LmFPPS mutant T164Y in complex with 476A, IPP & Ca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.525, 85.889, 107.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.980 - 1.800 |
| R-factor | 0.1603 |
| Rwork | 0.158 |
| R-free | 0.19940 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4jzx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.658 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.081 | 0.056 | 0.688 |
| Total number of observations | 422649 | ||
| Number of reflections | 67996 | 7308 | 5490 |
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 97.5 | 99.7 | 79.4 |
| Redundancy | 6.2 | 7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 100 mM MES, 15-25 % PEG 8000, 100-200 mM CaAcetate |
