6VIT

The Crystal Structure of Apo Domain-Swapped dimer Q108K:T51D:I32C Variant of HCRBPII with an Engineered Disulfide Bond

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-17
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97624
Spacegroup name	P 63
Unit cell lengths	133.283, 133.283, 51.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.480 - 3.200
R-factor	0.2388
Rwork	0.233
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.200	1.100
Rmeas	0.210
Number of reflections	8637	843
<I/σ(I)>	13.85	2.1
Completeness [%]	99.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	citric acid, PEG 3350