6VIP

TUDOR DOMAIN OF TUMOR SUPPRESSOR P53BP1 WITH MFP-6008

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	57.887, 60.156, 69.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.550 - 1.360
R-factor	0.1995
Rwork	0.199
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rg2
RMSD bond length	0.009
RMSD bond angle	1.570
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.380
High resolution limit [Å]	1.360	3.690	1.360
Rmerge	0.063	0.054	0.804
Rmeas	0.068	0.058	0.864
Rpim	0.025	0.021	0.313
Total number of observations	406505
Number of reflections	52885	2863	2597
<I/σ(I)>	7.9
Completeness [%]	100.0	99.8	100
Redundancy	7.7	7.2	7.5
CC(1/2)		0.997	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	2M ammonium sulfate, 0.1 M HEPES pH 7.5, 2% PEG 400