6VIP
TUDOR DOMAIN OF TUMOR SUPPRESSOR P53BP1 WITH MFP-6008
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.887, 60.156, 69.721 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.550 - 1.360 |
| R-factor | 0.1995 |
| Rwork | 0.199 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rg2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.380 |
| High resolution limit [Å] | 1.360 | 3.690 | 1.360 |
| Rmerge | 0.063 | 0.054 | 0.804 |
| Rmeas | 0.068 | 0.058 | 0.864 |
| Rpim | 0.025 | 0.021 | 0.313 |
| Total number of observations | 406505 | ||
| Number of reflections | 52885 | 2863 | 2597 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 7.7 | 7.2 | 7.5 |
| CC(1/2) | 0.997 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2M ammonium sulfate, 0.1 M HEPES pH 7.5, 2% PEG 400 |
