6VIJ
Crystal structure of mouse RABL3 in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.007 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.910, 78.203, 118.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.020 - 1.950 |
R-factor | 0.1955 |
Rwork | 0.194 |
R-free | 0.22700 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.947 | 5.290 | 1.950 |
Rmerge | 0.079 | 0.029 | 2.132 |
Rmeas | 0.081 | 0.030 | 2.201 |
Rpim | 0.019 | 0.007 | 0.541 |
Number of reflections | 37652 | 2045 | 1827 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 99.0 | 97.8 | 99.3 |
Redundancy | 17.8 | 17 | 15.8 |
CC(1/2) | 1.000 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Magnesium chloride hexahydrate, 12% w/v PEG 3350 |