6VIH

The ligand-free structure of mouse RABL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.998, 79.200, 111.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.313 - 2.993
R-factor	0.2313
Rwork	0.227
R-free	0.27500
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.095	0.075	3.711
Rmeas	0.098	0.077	3.815
Rpim	0.024	0.019	0.877
Total number of observations	205296
Number of reflections	11507	655	546
<I/σ(I)>	7.7
Completeness [%]	99.9	99.5	100
Redundancy	17.8	15.1	18.7
CC(1/2)		0.996	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.5 M Ammonium phosphate monobasic