6VID

The Crystal Structure of Aps Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Variant of HCRBPII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	67.050, 72.680, 164.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 2.890
R-factor	0.2515
Rwork	0.245
R-free	0.30780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.005
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.282	2.994
High resolution limit [Å]	2.891	2.891
Rmerge	0.170	0.115
Rmeas	0.178	1.316
Rpim	0.047
Number of reflections	18585	1745
<I/σ(I)>	2.4
Completeness [%]	99.4
Redundancy	7.7
CC(1/2)		0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, Sodium acetate, Ammonium acetate