6VI1

Structure of Fab4 bound to P22 TerL(1-33)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	77.747, 139.334, 163.761
Unit cell angles	90.00, 98.30, 90.00

Refinement procedure

Resolution	14.980 - 2.400
R-factor	0.1895
Rwork	0.189
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eu7
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.085	0.555
Number of reflections	127623	12764
<I/σ(I)>	11.3	2.3
Completeness [%]	94.0
Redundancy	2.5
CC(1/2)		0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	292	100 mM KCl, 25 mM MgCl2, 50 mM Na-Cacodylate trihydrate pH 6.0, 15% (v/v) 2-propanol