6VHP

Wild type EGFR in complex with LN2899

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	I 2 3
Unit cell lengths	145.811, 145.811, 145.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.970 - 3.600
R-factor	0.1839
Rwork	0.182
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d8e
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.970	38.970	3.690
High resolution limit [Å]	3.600	16.100	3.600
Rmerge	0.192	0.068	0.592
Rmeas	0.216	0.078	0.666
Rpim	0.097	0.037	0.300
Total number of observations	30048	317	2261
Number of reflections	6128	76	449
<I/σ(I)>	6.1	7.7	3.1
Completeness [%]	99.9	93.8	100
Redundancy	4.9	4.2	5
CC(1/2)	0.978	0.997	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.4M Sodium Citrate, 0.1 M MES