6VHN
Wild type EGFR in complex with LN2057
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 2 3 |
Unit cell lengths | 146.865, 146.865, 146.865 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.960 - 2.400 |
R-factor | 0.1989 |
Rwork | 0.199 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d8e |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.970 | 59.970 | 2.460 |
High resolution limit [Å] | 2.400 | 10.740 | 2.400 |
Rmerge | 0.106 | 0.067 | 0.683 |
Rmeas | 0.118 | 0.074 | 0.757 |
Rpim | 0.049 | 0.030 | 0.318 |
Total number of observations | 102763 | 1275 | 7659 |
Number of reflections | 20144 | 239 | 1483 |
<I/σ(I)> | 8.3 | 17.1 | 2.1 |
Completeness [%] | 97.7 | 93.5 | 99 |
Redundancy | 5.1 | 5.3 | 5.2 |
CC(1/2) | 0.992 | 0.994 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.4M Sodium Citrate, 0.1 M MES |